FIGURE 1.

Functional test of cell lines stably expressing FLAG–HDAg-S (Northern analysis). Cells were transfected with an HDV plasmid with either wild-type HDAg-S, or a mutant version of HDAg-S (mut HDAg) that does not support HDV replication (see Materials and Methods). RNA was isolated 4 d after transfection, and genomic HDV RNA was assessed by Northern blot. Results for four different clones 1–4 made with the same C-terminal Flag-tagged HDAg-S construct and a representative clone made with the N-terminal Flag-tagged HDAg-S are shown.