FIGURE 2.

Characterization of the oligomeric state of She2p at a physiological concentration range. Size-exclusion chromatography was performed to assess the oligomeric states of wild-type She2p (A) and She2p (L130→Y) (B). Whereas wild-type She2p forms a distinct oligomeric complex corresponding to a tetramer at concentrations up to 3.5 μM, the mutant She2p (L130→Y) shows impaired oligomerization over the entire concentration range. In addition to the protein concentration loaded, the estimated protein concentration after sample dilution on the column is listed. The real average concentrations might be even lower. Molecular weights were calculated from peak elution volumes. (C) Graph summarizing results from (A) and (B) using the estimated sample concentration on the column. Dashed lines indicate either the molecular weight of a She2p dimer (57 kDa) or of a tetramer (114 kDa).