FIGURE 4.

Real-time fluorescence-based analysis of RNA hydrolysis by a catalytic mutant of an exoribonuclease. FAM-linked RNA1 hydrolysis by RNase J1 or by a catalytic mutant of RNase J1 (Mathy et al. 2007) is analyzed by the release of a fluorescence signal. The experiment was performed in similar conditions to those in Figure 1. RT-FeDEx analysis demonstrates that a catalytic mutant of RNase J1 is unable to produce an increase in a fluorescence signal.