Figure 3.

In vivo lineage potential of Ly6d⁻ and Ly6d⁺ bone marrow progenitors by intrathymic transplantation. (A) Intrathymic transplants (i.t.) of MPPs, ALPs, BLPs, and pre-pro-Bs (ppB) into nonirradiated recipients. Similar proportions of cells were transplanted as in Figure 2. Thymuses were harvested at day 9, and the distribution of donor cells within each lineage is shown. Displayed are the combined results from two independent experiments. (B) Absolute number of donor T-cell output (left) and B-cell output (right) recovered from the intrathymic transplants shown in A. (C) Distribution of donor T-lineage cells from MPPs, ALPs, and BLPs, listed as a fraction of total donor T cells. Representative stains for intrathymic analyses and definitions of all lineages can be found in Supplemental Figure S7.