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Purification of a HARP-containing complex. Nuclear extracts were prepared from normal HeLa S3 cells (−) or HeLa cells expressing Flag-HA-tagged HARP (+). The proteins were subjected to sequential anti-Flag and anti-HA immunoaffinity purification, and then analyzed by SDS–polyacrylamide gel electrophoresis and silver staining. The polypeptides that consistently copurified with Flag-HA-HARP were identified by mass spectroscopy and are indicated.
