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. 2009 Oct 15;23(20):2400–2404. doi: 10.1101/gad.1831509

Figure 4.

Figure 4.

Wild-type but not RPA-binding-defective mutant HARP localizes to laser-induced damage sites. (A) Endogenous HARP protein is localized to sites of DNA damage in several different cell lines. The indicated cells were microirradiated with a nanosecond green laser. After 1 h, the cells were fixed and stained with anti-HARP antibodies. The nuclei are outlined with a dotted line. (B) Cells containing wild-type or mutant Flag-HA-HARP were microirradiated, as in A. After 30 min, cells were fixed and stained with anti-HARP and anti-Flag antibodies. The anti-HARP antibodies recognize both the endogenous and transgenic HARP, whereas the anti-Flag antibodies recognize only the transgenic HARP. All of the wild-type HARP-containing cells (N = 8) exhibited specific localization of HARP to the DNA damage sites, whereas none of the mutant HARP-containing cells (N = 8) showed localization of the mutant HARP to the DNA damage sites. (C) A simple view of HARP protein.