Figure 2.
Histology of mammary tissue of Stat5a/5bfl/fl;Cre;iStat5 mice in the presence or absence of doxycycline. (A) Schematic design of the experimental protocol. Mice were fed with doxycycline-containing food from mating to the end of the second lactation, and again after delivery of the fourth litter. (B) Mammary glands of Stat5a/5bfl/fl;Cre;iStat5 mice were biopsied on the day of delivering the first (panels a,d), fourth (panels b,e), and sixth (panels c,f) litters and were stained with hematoxylin and eosin (panels a–c) or immunostained with anti-phosphorylated STAT5 antibody (red) and anti-E-cadherin antibodies (green) (panels d–f). Black arrows indicate lumina. White arrows point to cells containing nuclear pSTAT5.