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. Author manuscript; available in PMC: 2009 Oct 20.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2007 Mar 9;293(1):L52–L59. doi: 10.1152/ajplung.00256.2006

Fig. 2.

Fig. 2

Experimental protocol. Following surgical preparation, rewarming to 36°C, and application of CPAP, 150 ml of isotonic instillate with 5% BSA was instilled into the air space, and 1 μCi of 125I-labeled albumin was added to the perfusate. Five minutes later, baseline samples of both air space fluid and perfusate were collected. Samples were taken again 30 min later. Immediately following the measurement of basal air space fluid clearance (AFC), a second volume of fluid containing terbutaline (10−5 M) was instilled into the lung. Air space and perfusate samples were subsequently collected at 5 min and 30 min for determination of AFC and epithelial albumin flux. Lung weight gain was measured from the start of the first measurement of AFC to the end of the protocol.