Figure 4. MC-TNF is required to mobilize DCs to DLNs after footpad challenge with E. coli.

(A) Total number of CD11c⁺/MHC Class II⁺ DCs in single cell suspensions of DLN at 12, 24, or 48 hours after saline or E. coli challenge. N≥10 DLNs for each time-point. ^*p<0.05 compared to saline controls. ^**p<0.05 comparing the 24 hour to the 12 and 48 hour E. coli challenged samples. (B) Total number of DCs in single cell suspensions of DLNs of WT, W/W^v, and WT MC-footpad repleted W/W^v mice 24 hours after challenge with saline or E. coli. N=6 for WT and W/W^v samples. N=4 for MC-repleted W/W^v samples. ^*p<0.05 compared to saline controls. ^**p<0.05, comparing E. coli challenged MC sufficient samples to the E. coli challenged W/W^v samples. (C) Total number of DCs in single cell suspensions of DLNs of WT and TNF^−/− mice 24 hours after challenge with saline or E. coli. N=4 for each sample. ^*p<0.001 compared to saline controls. ^**p<0.001 comparing E. coli challenged samples. (D) Total number of DCs in single cell suspensions of DLNs of WT-MC repleted W/W^v and TNF^−/− MC repleted W/W^v mice 24 hours after challenge with saline or E. coli. N=4 for each sample. ^*p<0.001 compared to saline controls. ^**p<0.001 comparing E. coli challenged samples. Data in A–D was analyzed for statistical significance with one-way ANOVA and Tukey’s post-test. Graphical bars represent the mean ±S.D.