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. 1998 May 26;95(11):6245–6250. doi: 10.1073/pnas.95.11.6245

Figure 2.

Figure 2

Interaction of LexA-Reg1 with Snf1 fusion proteins in the two-hybrid system. LexA-Reg1 and the indicated activation domain fusion to Snf1 sequence were expressed from the ADH1 promoter from plasmids listed in Table 1. Transformants of CTY10–5d were grown in selective SC + 4% glucose medium (high glucose, ░⃞). To obtain derepressed cells (low glucose, ▪), transformants were shifted to SC + 0.05% glucose for 3 to 4 h, except that transformants expressing GAD-Snf1KDK84R and GAD were grown in SC + 2% raffinose/0.05% glucose. Values are the average β-gal activity of four to six transformants. In control experiments, values for VP16-Snf1K84R and GAD-Snf1KDK84R in combination with LexA were <1. The increased interaction detected in derepressed cells is not due to increased expression of LexA-Reg1 (27) or Snf1 hybrid proteins (ref. 23 and data not shown).