Figure 3.

Snf1KD regulates SUC2 and GAL1 independently of Reg1. Strains MCY3922 (snf1Δ GAL1-lacZ) and MCY3923 (snf1Δ reg1Δ GAL1-lacZ) were transformed with pKL3, pRJ93, or the vector pRS424. pKL3 and pRJ93 express Snf1 residues 1–309 (here referred to as Snf1KD; elsewhere Snf1KD comprises residues 1–392) and the entire Snf1, respectively, from the SNF1 promoter. Transformants were grown to mid-log phase in selective SC + 2% glucose/2% galactose (Glu/Gal, ░⃞) and were shifted to SC + 0.05% glucose/2% galactose for 6 hr (Gal, ▪). Values are average invertase and β-gal activities for four transformants. ND, not determined because these cultures grew poorly.