Table 1.
Summary of mutation data
| Dose, Gy | Stage, weeks | No. of litters | No. of offspring | Number of mutations scored by different probes
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Grand total* | Multilocus probes only
|
Single-locus probes†
|
|||||||||||
| Total | 33.15+ MMS10 | MMS10 only | 33.15 only | Total | χ2 df = 1‡ | P-value§ | Ms6-hm | Hm-2 | |||||
| 0 | – | 8 | 54 | 20 (16) | 9 | 1 | 7 | 1 | 11 (6 + 5) | 0.09 | 0.7679 | 8 (4 + 4) | 3 (2 + 1) |
| 0.5 | Spermatogonia (6) | 8 | 45 | 26 (23) | 8 | 0 | 8 | 0 | 18 (12 + 6) | 1.98 | 0.1590 | 12 (7 + 5) | 6 (5 + 1) |
| 0.5 | Stem cells (10) | 8 | 55 | 41 (36) | 19 | 2 | 16 | 1 | 22 (16 + 6) | 4.61 | 0.0318 | 15 (12 + 3) | 7 (4 + 3) |
| 0.5 | 6 + 10 weeks | 16 | 100 | 67 (59) | 27 | 2 | 24 | 1 | 40 (28 + 12) | 6.50 | 0.0108 | 27 (19 + 8) | 13 (9 + 4) |
| 1 | Spermatids (3) | 10 | 62 | 21 (19) | 7 | 0 | 7 | 0 | 14 (10 + 4) | 2.56 | 0.1093 | 7 (3 + 4) | 7 (7 + 0) |
| 1 | Spermatogonia (6) | 12 | 73 | 60 (53) | 20 | 0 | 18 | 2 | 40 (32 + 8) | 15.23 | 0.0001 | 26 (22 + 4) | 14 (10 + 4) |
| 1 | Stem cells (10) | 5 | 25 | 21 (20) | 6 | 0 | 6 | 0 | 15 (12 + 3) | 5.60 | 0.0180 | 7 (5 + 2) | 8 (7 + 1) |
| 1 | 6 + 10 weeks | 17 | 98 | 81 (73) | 26 | 0 | 24 | 2 | 55 (44 + 11) | 21.01 | 0.0000 | 33 (27 + 6) | 22 (17 + 5) |
| Total | 51 | 314 | 189 | 69 | 3 | 62 | 4 | 120 (88 + 32) | — | — | 75 (53 + 22) | 45 (35 + 10) | |
| Fraction, % | — | — | — | 36.5 | 1.6 | 32.8 | 2.1 | 63.5 | — | — | 39.7 | 23.8 | |
*
Number of independent mutations are given in parentheses.
†
Numbers of paternal and maternal mutations are given in parentheses.
‡
χ2 test for equal number of paternal and maternal mutations.
§
Probability for χ2 test; statistically significant values are in bold.