Table I. KD values for pMHC tetramer and monomer binding.
A, KD values [M] determined for pMHC tetramer binding to OT-I wildtype or α-CPM mutant CD4+CD8+ DP thymocytes. ΔKD represents KD α-CPM mutant / KD wildtype. B, KD values determined for pMHc monomer binding to T1 wildtype, α-CPM mutant or TM control CD4+CD8+ DP thymocytes Mean values of three experiments are shown. ΔKD represents KD α-CPM mutant / KD TM control.
| A | Tetramer ligand |
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|---|---|---|---|---|---|
| Strain: | OVA-Kb | Q4R7-Kb | T4-Kb | Q4H7-Kb | |
| OT-I wt | 4.4 × 10−9 | 4.5 × 10−8 | 5.5 × 10−8 | 5.0 ×10−8 | |
| α-CPM cut | 1.7 × 10−8 | 1.4 × 10−7 | 2.1 × 10−7 | 2.0 × 10−7 | |
| ΔKD (KD α-CPM / KD wt) | 3.9 x | 3.1 x | 3.8 x | 4.0 x | |
| B | Monomer ligand |
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|---|---|---|---|---|
| Strain: | 4L-Kd | 4P-Kd | 4P-Kd Δ223/227 | |
| T1 wt | 2.8 ×10−8 | 8.8 ×10−8 | 1.4 ×10−6 | |
| α-CPM mut | 1.5 ×10−7 | 3.9 ×10−7 | 1.4 ×10−6 | |
| TM control | 3.5 ×10−8 | 9.5 ×10−8 | 1.7 ×10−6 | |
| ΔKD (KD α-CPM / KD TM ctrl.) | 4.3 x | 4.1 x | 0.8 x | |