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Residual tryptic- and caspase-like activities in purified 20S from EL-4-adapted cells. Purified EL-4 20S incubated with (A) 20 μM LLVY-AMC, (C) 20 μM LLR-AMC, or (E) 100 μM YVAD-AMC. Purified EL-4 adapted 20S incubated with (B) 20 μM LLVY-AMC, (D) 20 μM LLR-AMC, or (F) 100 μM YVAD-AMC. Reactions were incubated with no inhibitor (●), 50 μM NLVS (○), 50 μM YL3VS (□), 50 μM NLVS + 50 μM YL3VS (▵), or 50 μM lactacystin (⋄). Note scale differences for panels.
