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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Glia. 2009 Dec;57(16):1706–1715. doi: 10.1002/glia.20882

Figure 1. Generation and initial characterization of Kir4.1-EGFP mouse line.

Figure 1

(A) Schematic representation of the transgene Kir4.1-EGFP. The 185 kb mouse genomic bacterial artificial chromosome (BAC) clone RP23 – 157J4 containing the entire transcriptional unit of Kir4.1 together with 132 kb upstream and 52 kb downstream was engineered to harbor EGFP coding sequences followed by a polyadenylation signal (pA) into the coding region of Kir4.1 gene by homologous recombination in E. coli. (B) Intrinsic EGFP signal in coronal section of Kir4.1-EGFP mouse brain. (C–H) Intrinsic EGFP signals of Kir4.1-EGFP mouse brain in dentate gyrus of hippocampus (C), area CA1 of hippocampus (D), cortex (E), cerebellum (F-G), and olfactory bulb (H). (I–J) Western blot analyses of Kir4.1 channel expression in Kir4.1-EGFP and wild-type mouse brains. Arrows indicate the bands corresponding to Kir4.1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Kir4.1 knockout (KO) mouse brain was used as a control for the specificity of anti-Kir4.1 antibody used (I). Similar levels of Kir4.1 channel expression was verified using densitometric analyzes (n=5)(J). Scale bars: 1 mm (B), 50 μm (C,D,E,G,H) 100 μm (F).