Figure 2. EGFP is not expressed in neurons and co-localizes with Kir4.1 in brain.
(A–C) Immunostaining for EGFP and NeuN in the Kir4.1-EGFP cortex. Notice the expression of EGFP (arrows) in small cell bodies and lack of expression in NeuN+ cells (arrowheads). (D–I) Immunostaining for EGFP and Kir4.1 in area CA1 of the hippocampus (D–F) and cortex (G–I). Small squares are shown enlarged in insets in the lower left corners of each panel. Arrowheads indicate EGFP-positive cells that clearly show Kir4.1 expression in the soma. Scale bars: 50 μm (A,D,G).