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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Aug 27;1793(11):1787–1794. doi: 10.1016/j.bbamcr.2009.08.009

Fig 1. Downregulation of LH receptor mRNA in the post-mitochondrial supernatants and ribosomes of pseudopregnant rats injected with hCG.

Fig 1

Fig 1

Fig 1

Rats were injected with hCG on the fifth day of pseudo-pregnancy, ovaries were collected 0, 2, 4, 6 and 12 h later and polysomes were isolated. Total RNA from the control (CTL) or hCG-treated post-mitochondrial supernatant (S10) were reverse transcribed, and the resulting cDNAs were subjected to real-time PCR using predesigned primers and probes for rat LH receptor mRNA (A). Total RNA from the control (CTL) or hCG-treated polysomes were reverse transcribed, and the resulting cDNAs were subjected to real-time PCR using predesigned primers and probes for rat LH receptor mRNA (B), β–actin, GAPDH and RPS6 (C), as described in Materials and Methods. The graphs represent changes in mRNA levels normalized to 18S rRNA and shown as fold change vs. control. Error bars represent mean ±SE. *p<0.05, n=3.