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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Aug 27;1793(11):1787–1794. doi: 10.1016/j.bbamcr.2009.08.009

Fig 4. LH receptor mRNA co-precipitates with LRBP in the ribosomes from downregulated ovaries.

Fig 4

Polysomes prepared from 0 h control (CTL) and downregulated (hCG) pseudopregnant rat ovaries collected 2, 4, 6 and 12 h after hCG treatment were subjected to immunoprecipitation using LRBP antibody. Total RNA extracted from the immune complex was reverse transcribed and the cDNAs were used for real-time PCR using pre-designed primers and probes for rat LH receptor mRNA. The graph represents changes in LH receptor mRNA levels normalized to 18S rRNA and shown as fold change vs. control. Error bars represent mean ±SE. *p<0.05, n=3.