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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Aug 27;1793(11):1787–1794. doi: 10.1016/j.bbamcr.2009.08.009

Fig 7. in vitro translation of LH receptor mRNA; Effect of Polysome-associated LRBP.

Fig 7

Fig 7

200 ng of FLAG-tagged rat LH receptor mRNA (A) was in vitro translated using 15 μCi of [35S] methionine in the presence of ribosomes from control (CTL) and 4 h hCG-injected (hCG) ovaries. The translated LH receptor protein was immunoprecipitated and subjected to SDS-PAGE. The gel was dried and exposed to X-ray film for autoradiography. The experiment was repeated 4 times with the same results. The lower panel shows the quantitative expression of LHR. The graph represents the mean of four experiments and error bars represent mean ±SE. *p<0.05, n=4. B. in vitro translation was conducted in the presence of ribosomes from control (CTL) and 4 h hCG-treated (hCG) ovaries in the absence of any exogenously added mRNA (Lanes 1 & 2) or by using human β-actin mRNA (100 ng; Lanes 3 & 4). Lane 5 was β-actin mRNA (200 ng) without polysomes. Translated proteins were directly subjected to SDS-PAGE and autoradiography.