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. 2009 Oct 29;4(10):e7646. doi: 10.1371/journal.pone.0007646

Figure 4. Flagella and flagellin genes of Edwardsiella strains.

Figure 4

A. E. tarda strains WY37 (isolated from turbot), ATCC15947 (isolated from human feces) and ETV (isolated from human) were overnight cultured on LB liquid medium. Cells were collected by centrifugation at 1,000 rpm for 2 min following removing supernatant and then fixed with 2.5% glutaraldehyde. Scale bars represent 1 µm. B. The aligned putative flagellin sequences from E. tarda EIB202 (ETAE_2128, ETAE_2130), E. tarda PPD130/91 (AAN52540), E. ictaluri str. 93-146 (gi|238920295|, gi|238920297|, gi|238920300|) and S. typhimurium LT2 (NP_461698, NP_460912). Protein sequences are typically highly conserved at their C-terminal and N-terminal ends encoding the flagellar filament backbone while the middle region is generally quite variable, representing the surface-exposed and antigenically variable portion of the filament.