Figure 3. Effect of the t(9;22) fusion proteins on the re-population capacity of murine HSC.

(A) The schematic indicates the experimental procedure for determination of the re-population potential of murine HSCs expressing t(9;22) fusion proteins. Sca1⁺/lin⁻ BM cells from CD45.1⁺ mice were infected with the indicated retroviruses and, at 48 h post-infection, the cells were transplanted together with CD45.2⁺ BM cells into lethally (11 Gy) irradiated CD45.2⁺ recipient mice. Analysis of donor chimerism was performed 12 weeks after transplantation. (B) Plots show representative donor-derived chimerisms from individual mice at 12 weeks post-transplantation. (C) Graph of donor-derived chimerism in each mouse after long-term reconstitution (4–5 mice/group).