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. 2009 Oct 7;106(42):17858–17863. doi: 10.1073/pnas.0903474106

Fig. 5.

Fig. 5.

PVR and PVRL2 but not PVRL3 are ligands for TIGIT. (A) Flow cytometry analysis of 721.221 parental cell line and the various transfectants stained with the indicated mAbs and with the fusion proteins TIGIT-Ig and DNAM1-Ig (black lines). Gray filled histograms are the background staining with the secondary fluorescein- (for mAbs) or phycoerythrin- (for fusion proteins) conjugated antibody only. The parental 721.221 cells were stained by all three anti-PVR, PVRL2 and PVRL3 antibodies (B) Mouse IL-2 secretion from parental BW cells or BW cells expressing the TIGIT ζ chimeric protein, incubated for 48 h with the 721.221 transfectants presented in A. Mouse IL-2 secretion was measured by ELISA. (C) Killing of the indicated 721.221 transfectant cells by YTS/TIGIT and YTS/TIGIT Y231stop cells. The E:T ratios are indicated in the x axis.