Fig. 6.

TIGIT protects normal cells expressing PVR and PVR-like proteins from NK cell-mediated killing. (A) Primary fibroblasts characterization. Flow cytometry analysis of primary human foreskin fibroblast (HFF) cells stained with the indicated mAbs and fusion proteins (black lines). Gray filled histograms are the background staining with the secondary fluorescein- (for mAbs) or phycoerythrin- (for fusion proteins) conjugated antibody only. (B) Killing of the primary human foreskin fibroblasts by primary NK cells at an E:T ratio of 20:1. NK cells were either preincubated or not with anti-TIGIT mAb #5 or with no mAb. Fibroblasts were preincubated either with no fusion proteins or with the indicated fusion proteins.