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. 2009 Oct 1;106(42):17910–17915. doi: 10.1073/pnas.0909353106

Fig. 6.

Fig. 6.

ACF inhibits tumor growth, HIF-1 target gene expression, and HIF-1 activity. (A and B) PC-3 xenografts were grown to approximately 100 mm3 and mice were treated by daily i.p. injection of vehicle or ACF for 14 days. (A) Tumor volume (mean ± SEM; n = 4) is shown. *, P < 0.05; **, P < 0.01 vs. vehicle (two-way ANOVA with Bonferroni correction). (B) Mice were euthanized on day 28 (4 h after last dose) and tumors were collected. mRNA levels relative to 18S rRNA in tumors from vehicle- and ACF-treated mice were calculated as 2−Δ(ΔCt), where ΔCt = Cttarget − Ct18S and Δ(ΔCt) = Δ Ctvehicle − ΔCtACF. Mean ± SEM (n = 4) is shown. *, P < 0.05; **, P < 0.01 (two-way ANOVA with Bonferroni correction). (C-D) mice bearing Hep3B-c1 xenografts were treated with vehicle or ACF starting on day 25. (D) HRE-driven FLuc activity was determined by Xenogen imaging before treatment (Upper) and 4 h after treatment on day 28 (Lower).