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. 2009 Oct 6;106(42):17687–17692. doi: 10.1073/pnas.0905073106

Fig. 2.

Fig. 2.

Purification of BamBC and activity staining. A SDS-polyacrylamide gel of dienoyl-CoA:acceptor oxidoreductase activity containing fractions obtained during the purification of BamBC from G. metallireducens, 7 μg of protein per lane. Lane 1, molecular mass standard; lane 2, cell extract; lane 3, dialysed extract; lane 4, DEAE Sepharose; lane 5, Cibacron Blue and Reactive Red agarose; lane 6, hydroxyapatite; lane 7, SourceQ Sepharose. (B) Native gel electrophoresis and activity staining. Lane 1, BSA standard (Coomassie-stained); lane 2, soluble extract of cells grown benzoate; lane 3, purified BamBC. Lanes 2 and 3 were activity-stained. (C) SDS polyacrylamide gel analysis of bands activity-stained bands (B3.1 and B3.2). Lane 1, molecular mass standard; lane 2, excised band at 180 kDa in B; lane 3, excised band at 100 kDa in B.