Table 1.

Protocol for purification of BamBC

Purification step	Volume, mL	Total activity, U	Protein, mg	Specific activity, U mg−1	Yield, %	Enrichment, fold
100,000 × g supernatant	79	1,778	2,955	0.6	100	1.0
Dialysis	80	2,102	2,880	0.7	118	1.2
DEAE	310	1,273	322	3.9	71	6.6
CibacronBlue/ReactiveRed	320	615	102	6.0	35	10
Hydroxyapatite	50	452	22	20	26	33
SourceQ	24	321	6.0	68	18	115

One U refers to the oxidation of 1 μmol min⁻¹ dienoyl-CoA. Activities were determined with DCPIP as electron acceptor.