Fig. 1.
Crosses to measure Y-linked rDNA deletions and test their effect on gene expression. (A) Males harboring a YrDNA-deletion (First row, middle, “YrDNA-del”) were crossed to females carrying the compound C(1)DX chromosome (first row, left), which lacks rDNA, or to females that harbor the chromosomal inversion In(1)wm4 (first row, right). Female progeny (second row, left) were used to measure rDNA quantity genetically and molecularly, and male progeny (second row, right) were used to measure expression of white. (B) Categories of white expression and quantification of pigment. The YrDNA-deletion along with an In(1)wm4 chromosome produced male progeny with three categories of white expression. Eyes showing representative pigmentation from categories used for scoring (cat 1, cat 2, cat 3), and quantification of the pigment extracted from members of each category (+S.D.).