Fig. 4.
Comparison between rRNA concentration and nucleolar volume and morphology between wild-type and YrDNA-deletion flies. (A) Comparison of rRNA between adults of genotype wm4/YrDNA-deletion, relative to Ywt (which is defined as 100%), black bars, mean (±S.D.). Values seem elevated, but not significantly so (Student's t-test): Ywt vs. YrDNA-0.87 (P = 0.304), Ywt vs. YrDNA-0.87 (P = 0.111). (B) Quantification of nucleolar volume as fraction of total nuclear volume from 3-D reconstructed salivary gland nuclei of wild-type (Ywt), YrDNA-0.87, and YrDNA-0.36 [n = 30 nuclei for each genotype, all data are shown, gray circles indicate the mean of combined data sets, Student's t-test: Ywt vs. YrDNA-0.87 (P < 0.001), YrDNA-0.87 vs. YrDNA-0.36 (P = 0.003)]. (C–E) confocal images of nuclei from C(1)DX/Ywt, YrDNA-0.87, and YrDNA-0.36 processed for immunofluorescence to fibrillarin (red) and stained with DAPI to reveal DNA (blue). Insets for YrDNA-0.87 and YrDNA-0.36 are increased magnification showing mininucleoli and micronucleoli in respective chromosome preparations. Panels show range of mininucleolar and micronucleolar phenotypes of YrDNA-deletion chromosomes.