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. 2009 Oct 1;106(42):17870–17875. doi: 10.1073/pnas.0909545106

Fig. 3.

Fig. 3.

Interaction of RIG-I with immunogenic DNAs in vitro. (A) In vitro B-DNA pull-down analysis of RIG-I. Recombinant GST-RIG-I and GST-RIG-IΔCARD were incubated with biotin-conjugated B-DNA and streptavidin (SA)-conjugated magnetic beads in the absence or presence of unconjugated B-DNA or poly(I:C). Bound proteins were analyzed by immunoblot analysis using anti-GST antibody (left panel). Input protein levels for this assay are shown in the right panel. (B) Interaction of intact RIG-I with B-DNA and other immunogenic DNAs. The same pull-down assay was performed by incubating GST-RIG-I with biotin-conjugated B-DNA in the presence of increasing amounts of the indicated DNAs. Note that the GST-RIG-I blot is exposed 100-fold longer than that shown in A to visualize the pulled down GST-RIG-I.