Fig. 3.

Processing and generation of intermediate-size transcripts. (A) Cells expressing an ectopic copy of Dcl1 under the control of a tet-inducible promoter were incubated with different tet concentrations (lanes 2 and 3) or without tet (lane 1). The expression of DCL1 was monitored by Western blotting (DCL1, Bottom). Low MW RNA was isolated and hybridized to a CIR147 probe. (B) The dcl1/2null cell line (lane 1) was complemented with dcl1 (dcl1c, lane 2) or dcl2 (dcl2c, lane 3), and low MW RNA isolated from the three cell lines was hybridized to a SLACS probe. Sizes are indicated in nucleotides.