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(A) Luciferase expression with the positive control plasmid. Expression from AM1-P was measured repeatedly in wild type cells for 42 h at 34°C. (B) Effect of amount of transfection plasmid DNA on luciferase expression. A range of plasmid quantities (10, 50, 100, 200, 400 and 800 ng) of the positive control (AM1-P, diamonds) or uracil-DNA (AM1-U, circles) plasmids were used for transfection, and the luciferase expression at the 10 h time point was plotted.
