Fig. 2.
Gonadotropin regulation of DDR2 expression in ovaries. DDR2 indirect immunoreactivity (brown) in cross-sections of ovaries from adult wild type (+/+) and Ddr2slie/slie mutant, counterstained with hematoxylin (violet), after saline (A, D), PMSG (B, E), or PMSG + hCG (C, F) treatments. DDR2 immunoreactivity was elevated after PMSG alone compared with saline and especially after PMSG + hCG in interstitial cells (open arrowheads) of the ovaries (B, C), whereas ovarian sections form Ddr2slie/slie mutants served as negative control (E, F). Immunoblot (G) and graph of relative optical density for LHR immunoreactive protein (H) harvested from extracts of whole ovaries from wild type upon either PMSG alone or PMSG + hCG treatment compared with saline control. O, oocytes, I, interstitial cells; PMSG, pregnant mare serum gonadotropin; hCG, human chorionic gonadotropin; LHR, luteinizing hormone receptor. Scale bar 100 μm.