Fig. 5.

Gene expression confirmation studies in ovarian tissue from wild-type (+/+) and Ddr2^slie/slie mutant mice. Gene expression is affected in Ddr2^slie/slie mutant ovaries at 10 wk of age after gonadotropin treatment. Reverse-transcriptase PCR (A, B) and qPCR (C) analysis confirmed some genes identified from transcriptome analysis: Ddr2, Mmp2 (matrix metallopeptidase 2), Mmp9 (matrix metallopeptidase 9), Mmp19 (matrix metallopeptidase 19), Mmp14 (matrix metallopeptidase 14), Lhcgr (luteinizing hormone/choriogonadotropin receptor), Fshr (follicle stimulating hormone receptor), Ptger2 (prostaglandin E receptor 2, subtype EP2), Ptgfr (prostaglandin F receptor), Pgr (progesterone receptor), and housekeeping gene Gapdh (glyceraldehyde-3-phosphate dehydrogenase, also known as G3PDH) in wild-type and Ddr2^slie/slie mutant ovaries after injection with PMSG and hCG. All data represent means and SE. Significant differences indicated by *P < 0.05, **P < 0.01. D–G: LHR (brown) protein levels were examined by indirect immunohistochemistry of ovarian tissue from wild type and Ddr2^slie/slie mutant after saline (D, F), and PMSG + hCG (E, G) stimulation. LHR immunoreactivity was decreased in interstitial cells of the Ddr2^slie/slie ovaries (arrowheads) after treatment to either saline alone or exogenous gonadotropins (F and G). O, oocytes. Scale bar 100 μm.