Table 3.
Relative Array and PCR expression of selected genes highly expressed in HCCEC compared to HCAEC and HUVEC
| Accession No. | Gene Name | Fold Δ vs. HCAEC |
Fold Δ vs. HUVEC |
Taqman Assay ID | ||
|---|---|---|---|---|---|---|
| Array | PCR | Array | PCR | |||
| NM_001792 | Cadherin 2* | 2.48 | 1.72 | 2.47 | −1.35 | Hs00169953_m1 |
| NM_001797 | Cadherin 11* | 2.12 | 5.11 | 1.97 | −1.16 | Hs00156438_m1 |
| NM_003277 | Claudin 5 | −2.16 | −5.81 | −2.21 | −1.9 | Hs00533949_s1 |
| NM_005602 | Claudin 11 | 5.03 | 25.44 | 4.42 | 2.71 | Hs00194440_m1 |
| NM_004415 | Desmoplakin* | 13.29 | † | 9.76 | † | Hs00189422_m1 |
| NM_004114 | FGF 13 | 5.32 | 8.88 | 4.05 | 16.85 | Hs00182807_m1 |
| NM_013372 | Gremlin 1* | 19.19 | † | 22.19 | † | Hs00171951_m1 |
| AI380298 | Syndecan 2* | 10.9 | 7.5 | 8.04 | 4.58 | Hs00299807_m1 |
| R94644 | Versican* | 8.39 | 25.6 | 4.21 | 4.48 | Hs01007937_m1 |
*
Array fold change in certain cases is an average because the gene had multiple probe sets called significant.
†
For Desmoplakin and Gremlin 1, CT values were low for human corpus cavernosum endothelial cells (HCCEC); for human coronary artery endothelial cells (HCAEC) and human umbilical vein endothelial cells (HUVEC) the amplification never reached the linear range, implying very low expression of the gene but making fold change calculations impossible.