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. 2009 Sep 29;106(41):17308–17313. doi: 10.1073/pnas.0900755106

Fig. 2.

Fig. 2.

CAPS stimulates full fusion at low t-SNARE densities. (A) v-SNARE and t-SNARE(10% PIP2) liposomes were incubated with 0.1 mM LPC for 10 min (filled squares) before lipid mixing reactions were conducted without additions (open circles) or with 1 μM CAPS (filled circles, filled squares). (B) Tb3+-loaded v-SNARE and DPA-loaded t-SNARE(10% PIP2) liposomes were prepared with 640 and 340 SNARE molecules/μm2, respectively, and incubated without additions (open circles), with 5 μM soluble syntaxin (open squares), with 1 μM CAPS (filled circles), or with CAPS plus soluble syntaxin (filled squares). Mixing of contents was expressed as percentage of complete mixing following detergent lysis. (C) Lipid mixing reactions were conducted with v-SNARE liposomes and t-SNARE(5% PIP2) liposomes that contained 1 to 100 copies of syntaxin-1/SNAP-25 in the absence (open circles) or presence of 1 μM CAPS (filled circles) (mean ± SD, n = 3; †Not different; *, P > 0.05 for with vs. without CAPS).