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A Gly-46 to Ser-46 sequence change in pinB is common among hard wheat varieties and is absent in soft wheat varieties. Gel photo (negative image) of PCR products obtained by using a serine-specific PCR primer on genomic DNA of soft and hard hexaploid wheat varieties. Soft (S) indicates that the variety has soft-textured grain, and hard (H) indicates hard-textured grain. Lane marked MW is a 1 kb molecular mass marker ladder.
