Figure 2.

PinA transcripts (Puro A) are absent in hard wheat varieties that lack the pinB Ser-46 mutation. PinB transcripts (Puro B) are present in all wheats irrespective of hardness. Each lane was loaded with 10 μg of total RNA isolated from immature seeds at 14 days after flowering. Replicate blots were probed with either pinA or B. SKHD and Class represent the Single Kernel Characterization System phenotypic grain hardness reading and hardness class, respectively (S, soft class; H, hard class).