Figure 4.

PinA transcripts (Puro A) are absent in the hard wheat variety Falcon (F) and two hard NILs derived from Falcon and Heron (H) soft wheat, one each, after 7 backcrosses (90267 and 90278). PinA transcripts are present in Heron and the two soft NILs (90259 and 90291). PinB transcripts (Puro B) are present in all wheats irrespective of hardness. Each lane was loaded with 10 μg of total RNA isolated from immature seeds at 14 days after flowering (DAF). Replicate blots were probed with either pinA or pinB. SKHD and Class are as described in Fig. 2.