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. Author manuscript; available in PMC: 2010 Apr 17.
Published in final edited form as: J Control Release. 2009 Jan 12;135(2):166–174. doi: 10.1016/j.jconrel.2008.12.016

Fig. 4. Plasmids/nucleus and luciferase expression dose response of PEI polyplexes and LFN lipoplexes.

Fig. 4

B16F10 (a–b) or A549 (c–d) cells were transfected with PEI/pLuc polyplexes or LFN/pLuc lipoplexes at the indicated doses. (a,c) Detected plasmids/nucleus in nuclei isolated by the iodixanol method. (b,d) Detected luciferase expression in lysates from transfected cells using the Promega Luciferase Assay kit normalized to the amount of total protein determined by the Bradford assay. Error bars represent the standard deviation of triplicates in the qPCR assay or luciferase assay.