FIGURE 10. Changes in the gene expression of the major sodium and chloride transport proteins by human alveolar epithelial type II cells by pretreatment with a p38 MAPK inhibitor prior to exposure to ALI pulmonary edema fluid.
Human alveolar epithelial type II cells cultured for 3 days were exposed to ALI pulmonary edema fluid or plasma for 24 h. Another set of human type II cells were pretreated with 10.0 μm SB202190 for 30 min prior to exposure with ALI pulmonary edema fluid. The gene expression was measured by two-step multiplex quantitative RT-PCR and expressed as the percent GCN of plasma ± S.D. of each sample measured in triplicates. *, p < 0.05 compared with plasma controls; †, p is significant by analysis of variance with Bonferroni correction. PE, pulmonary edema.