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. Author manuscript; available in PMC: 2009 Oct 21.
Published in final edited form as: J Biol Chem. 2007 Jun 19;282(33):24109–24119. doi: 10.1074/jbc.M700821200

FIGURE 4. A and B, effect of ALI pulmonary edema fluid on the gene expression of the major inflammatory cytokines and chemokines (A) and sodium and chloride transport proteins (B) by human alveolar epithelial type II cells exposed to ALI pulmonary edema fluid or plasma.

FIGURE 4

Human alveolar epithelial type II cells cultured for 3 days were exposed to ALI pulmonary edema fluid or plasma for 24 h. The gene expression was measured by two-step multiplex quantitative RT-PCR and expressed as the percent GCN of plasma ± S.D. of each sample measured in triplicates. *, p < 0.01 compared with plasma controls. The number above each cytokine/chemokine represents the multiple times the GCN of ALI pulmonary edema exposed cells exceeded the GCN of plasma exposed cells. PE, pulmonary edema.