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. 2009 Aug 14;75(20):6488–6495. doi: 10.1128/AEM.01055-09

TABLE 4.

Results of the validation hybridizations using DNA and RNA from 18 LAB strains based on oligonucleotide typea

Strain No. of species-specific oligonucleotides
No. of cross-hybridizing oligonucleotides
No. of unrelated oligonucleotides
Totalb DNAc RNAd Totalb DNAc RNAd Totalb DNAc RNAd
Lactobacillus curvatus D06SS01T01-H12 10 4 4 14 8 5 2,245 250 38
Lactobacillus fermentum 222 14 7 7 8 3 3 2,247 153 59
L. fermentum LMG 8154 14 7 7 8 4 3 2,247 147 2
L. fermentum IMDO 130101 14 7 7 8 2 2 2,247 218 51
Lactobacillus plantarum D06SS01T01-H18 302 256 243 50 36 29 1,917 283 35
L. plantarum 80 302 264 259 50 35 32 1,917 226 100
L. plantarum LMG 9211 302 286 279 50 33 31 1,917 176 89
L. plantarum ACA-DC 287 302 258 255 50 34 31 1,917 184 97
Lactobacillus sanfranciscensis LMG 16002T 28 25 25 7 6 6 2,234 155 12
Lactobacillus johnsonii La1 96 94 89 46 34 31 2,127 215 117
Lactobacillus acidophilus IBB 801 207 198 181 46 43 38 2,016 203 120
Lactobacillus delbrueckii subsp. delbrueckii LMG 6901T 117 81 75 26 15 11 2,126 213 66
Lactobacillus sakei subsp. sakei CTC 494 187 165 160 48 33 27 2,034 176 73
Lactococcus lactis subsp. lactis LDV 22186 551 386 344 30 18 14 1,688 137 59
Lc. lactis subsp. cremoris MG1363 551 342 280 30 20 17 1,688 100 45
Enterococcus faecium RSZ C5 113 85 70 27 7 7 2,129 157 77
Enterococcus faecalis LMG 8222 206 181 171 37 19 14 2,026 154 65
Leuconostoc mesenteroides subsp. mesenteroides LMG 6893 120 103 101 18 16 14 2,131 198 62
a

The oligonucleotide type was determined based on sequence annotation. Species-specific oligonucleotides were designed based on sequences of the corresponding LAB species; cross-hybridizing oligonucleotides were designed based on sequences from other LAB species and hybridized to the species indicated based on annotation; and the unrelated oligonucleotides did not show any homology to sequences from the species indicated.

b

Number of oligonucleotides that were available on the microarray.

c

Number of oligonucleotides whose intensities were above the background level for DNA hybridizations.

d

Number of oligonucleotides whose intensities were above the background level for RNA (a subset of the oligonucleotides whose intensities were above the background level for DNA).