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. 2009 Aug 21;75(20):6496–6503. doi: 10.1128/AEM.01669-09

FIG. 3.

FIG. 3.

PCR analysis of selected B. pseudomallei mutant strains isolated in this study. PCR was performed on colony boiling preparations (4) of the indicated strains with primers flanking the mutant allele (primer sequences available upon request). Lane 1, K 96243 wild-type purM locus; lane 2, Bp190 (K96243 ΔpurM); lane 3, 1026b wild-type bpeEF-oprC locus; lane 4, Bp253 [1026b Δ(bpeEF-oprC)]; lane 5, 1026b wild-type BPSS2307 locus; lane 6, Bp254 (1026b ΔBPSS2307). PCR products were separated on a 1% agarose gel and stained with ethidium bromide. Sizes of the expected PCR fragments (in bp) are indicated above the respective bands (the size of the expected DNA fragment for the wild-type bpeEF-oprC locus is indicated in parentheses, since it could not be amplified under the PCR conditions used here). Lanes M contained the Hi-Lo DNA ladder (Minnesota Molecular, Minneapolis, MN), and the sizes of pertinent fragments are indicated on the left.