Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
B. pseudomallei mutants generated with the I-SceI method
| Allele | Host straina | Mutant | Homology (bp)
|
Size of deletion (bp) | |
|---|---|---|---|---|---|
| Upstream | Downstream | ||||
| ΔpurM | 1026b | Bp318 | 742 | 689 | 114 |
| ΔpurM | K96243 | Bp190 | 742 | 689 | 114 |
| Δ(bpeEF-oprC) | 1026b | Bp253 | 769 | 813 | 4,465 |
| ΔBPSS2307 | 1026b | Bp254 | 824 | 763 | 1,517 |
All strains were generated using pEXKm4, except for Bp318 which was engineered using pEXKm5.
