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Mutant T1 contains an insertion of IS426 in a gene essential for the biosynthesis of 3-indoleacetic acid, iaaM. (A) Amplicons obtained with primer pair CYT21-CYT2. Nonpathogenic mutant bacteria T1 and P72 were isolated from tumors induced in tomato and pepper, respectively, by the wild-type strain C58. M, marker XVII (Life Technologies). (B) Position of IS426 in the T-DNA of mutant T1. The annealing sites of the different primers used for PCR and sequencing are indicated with arrows.
