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Presence of the T-DNA region in nonpathogenic mutants derived from IVIA1102. Plasmid DNA was digested with HindIII and separated on an agarose gel before Southern hybridization; the probe used was the complete T-DNA region from strain C58, obtained from plasmid pTHE17. P, parental strain IVIA1102; M, marker λHindIII (Invitrogen).
