TABLE 2.
Primers employed to examine possible indels responsible for the loss of pathogenicity in the mutants and for sequencing the insertion of mutant T1
| Primer | Sequence (5′-3′) | Size (bp) | Positiona | Reference |
|---|---|---|---|---|
| FGPtmr530 | CCA TGT TGT TTG CTA GCC AG | 172 | ||
| FGPtmr701 | CCT TCG AAT CCG TCG AAA GC | 39 | ||
| VirDA | ATG CCC GAT CGA GCT CAA GT | 338 | ||
| VirDE | CCT GAC CCA AAC ATC TCG GCT GCC CA | 16 | ||
| CYT1 | GAT CGG GTC CAA TGC TGT | 427 | ||
| CYT2b | GAT ATC CAT CGA TCC CTT | 19860 | 16 | |
| CYT21b | TCC ATC GCG TTT ACA GC | 2,449 | 17411 | This work |
| INS-1c | CGA GCA TCT CTC TGA CAA T | This work | ||
| INS-2c | ATG CTC GGT CGC AAG AC | This work | ||
| INS-3b | ACT GGC TTT ACC GTC TCC | 19234 | This work |
a
Position of each of the primers employed for sequencing the insertion of mutant T1, according to the sequence of pTi of strain C58 from Wood et al. (54), with GenBank accession number AE008690.
b
Primers employed for the sequencing of the insertion in mutant T1.
c
Primers designed from the insertion sequence of mutant T1. There is no correspondence with the sequences of T-DNA.