TABLE 4.
Mutant | Origin (strain, host) | Results of complementation analyses witha:
|
|||
---|---|---|---|---|---|
No plasmid | pVCK219b | pVCK225c | pTHE17d | ||
P72 | C58, pepper | − | − | − | − |
T1 | C58, tomato | − | − | − | + |
T22, T38, T67 | IVIA1102, tomato | − | − | − | + |
T32, T76 | IVIA1102, tomato | +d | +d | +d | + |
Results of the inoculation of transformed colonies in tomato. +, tumor; −, no tumor; +d, tumor smaller than that caused by the parental strain. The same results were obtained with six independent transformants per strain for each plasmid tested.
Plasmid containing virH, virA, and virB genes; see Fig. 1.
Plasmid containing virG, virC, virD, and virE genes; see Fig. 1. This plasmid plus pVCK219 covers the whole vir region of strain A6 (see Fig. 1).
Plasmid containing the entire T-DNA region from strain C58.