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. 2009 Aug 28;75(20):6568–6580. doi: 10.1128/AEM.01148-09

FIG. 4.

FIG. 4.

Temperature-dependent and strain-specific expression of the phzM gene and its regulatory genes lasI and ptsP determined by assaying β-Gal activity in Pseudomonas sp. strain M18 and P. aeruginosa PAO1. (A) Physical maps of phzM′-′lacZ translational fusion plasmid pPML and transcriptional fusion plasmid pPMC in strain M18 and pPMCO and ptsP′-′lacZ translational fusion plasmid pPPL in strain PAO1. lasI′-′lacZ translational fusion plasmid pLIL was constructed by the same method as in our previous work (4). SD, putative Shine-Dalgarno sequence. phzM and ptsP sequences are shown by heavy black lines, and lacZ sequences are shown by white boxes. PphzM and PptsP are the phzM and ptsP gene promoters. (B) β-Gal activities of M18/pPML (circles) and PAO1/pPML (squares) at 28°C (solid symbols) and 37°C (empty symbols) and strain M18 (diamonds) containing control plasmid pME6015. (C) β-Gal activities of M18/pPMC (circles) and PAO1/pPMCO (squares) at 28°C (solid symbols) and 37°C (empty symbols) and strain M18 (diamonds) containing control plasmid pME6522. (D) β-Gal activities of M18/pLIL (circles) and PAO1/pLIL (squares) at 28°C (solid symbols) and 37°C (empty symbols) and strain M18 (diamonds) and PAO1 (hexagons) containing control plasmid pME6015. (E) β-Gal activities of M18/pPPL (circles) and PAO1/pPPL (squares) at 28°C (solid symbols) and 37°C (empty symbols) and M18 (diamonds) and PAO1 (hexagons) containing control plasmid pME6015. Growth was in PPM broth. The values are means ± standard deviations of triplicate cultures.