TABLE 2.
Primers used in this study
| Primera | Nucleotide sequence | Restriction siteb |
|---|---|---|
| phzMsp1 | 5′-TGGAATGCCAGGTTGCTC-3′ | |
| phzMsp2 | 5′-CGCCGTAGAACAGCACCAT-3′ | |
| kan-F | 5′-CCGGGAGCTCGAATTCAGCAGAGCGAGGTATGTAGG-3′ | SacI |
| kan-R | 5′-CCGGGAGCTCGAATTCCAGGTGGCACTTTTCGGGGA-3′ | SacI |
| phzM1F | 5′-GGCCGAATTCTCTCCAGGTATGCCGGAGAA-3′ | EcoRI |
| phzM1R | 5′-GGCCAAGCTTGAAAGTTCCGATTCAGGCCC-3′ | HindIII |
| phzM2F | 5′-GGCCGAATTCGGTACTTCTCGGGTTACGGA-3′ | EcoRI |
| ptsP1 | 5′-CCGCGCGAATTCGACAGGTGGTGACCTTCAAG-3′ | EcoRI |
| ptsP2 | 5′-CCGCGC AAGCTT GAAATCAGGGCTGGACGGTA-3′ | HindIII |
| PM9F | 5′-GGCCGAATTCTCTCCAGGTATGCCGGAGAA-3′ | EcoRI |
| PM9R | 5′-CCGGCTGCAGCGCAGCAGCAAGATTCGAAT-3′ | PstI |
| DLasbF | 5′-GGCCGAATTC GCCAGACAGGGTATGCGGGA-3′ | EcoRI |
| TMPR | 5′-CCGGCTGCAG AGCATCAGCTTAGCAATCCC-3′ | PstI |
| TMPRO | 5′-CCGGCTGCAG AAGCATCAGCTTAGCAATCC-3′ | PstI |
| ptsPPF | 5′-GCGCGAATTCTGTATCCTCCCCGCACGAGT-3′ | EcoRI |
| ptsP620fR | 5′-CGGCCTGCAGGATCTTGCGCAGCGTGTTGA-3′ | PstI |
| RTphzM1 | 5′-GGATCGACAGCGACGAGACG-3′ | |
| RTphzM2 | 5′-CTCGCCGTAGAACAGCACCAT-3′ | |
| PSRMRPOD1 | 5′-GAGCGGGAGGAGCGTTTAC-3′ | |
| PSRMRPOD2 | 5′-CGGGCAAAAAATAAGCAGAGG-3′ | |
| RTlasI1 | 5′-AACTGCTGGGCGAGATGC-3′ | |
| RTlasI2 | 5′-CGCCAGCAACCGAAAACC-3′ | |
| RTptsP1 | 5′-AAGTGAACTCCGCCAAGG-3′ | |
| RTptsP2 | 5′-TGGAACGCTTGTTGAGGC-3′ |
a
All primers were obtained from Invitrogen (Shanghai, China).
b
The restriction sites used in the cloning procedure are underlined.